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Basic information
Hepatocyte growth factor regulates the development of highly pure cultured chief cells from rat stomach by stimulating chief cell proliferation in vitro.
We demonstrate a new technique to grow isolated chief cells in culture from the rat stomach that have ahigh TER, continuous tight junctions, low permeability, and stimulated pepsinogen secretion. Success of the procedure relies on using HGF, which is thought to be a potent scatter factor but facilitates monolayer development in chief cells by facilitating their proliferation. |