To confirm our previous findings suggesting the importance of early changes in gene expression in an artificial wound healing model using human keratinocyte cells (HaCaT), we further analyzed transcriptome (total mRNA population) and translatome (ribosome-bound mRNA). Artificially-injured confluent HaCaT were exposed to either an inhibitor of transcription (30-50 min-) or translation (5-25 min-period post-injury) and harvested for transcriptome or translatome analyses as below.Both inhibitors delayed wound healing. The transcriptome analyses against 54,359 probes showed expression changes (<0.5/>2.0-fold) of 1,170/1,397 and 1,634/3,678 genes, respectively, at 30 min and 6 hrs post-injury. Likewise, changed genes in the translatome analyses were 6,146/4,897 and 5,383/4,880 at 30 min and 3 hrs, respectively. The data suggest that transcribed genes increase but the number of mRNAs under translation decreases along wound healing. Changed genes were in a greater number in the translatome than in transcriptome in the time course of wound healing. This suggested that a substantial change in cellular translation might occur within 5-25-min post-injury. Elucidation of such changes would help developing new therapeutic strategies for efficient wound healing.
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