講演・口頭発表等[R]

基本情報

氏名 新垣 知輝
氏名(カナ) シンガキ トモテル
氏名(英語) Shingaki Tomoteru
所属 薬学部 医療薬学科
職名 准教授
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タイトル

Development of Detection method for D-Amino Acid Incorporation during in an Escherichia coli Cell-Free Translation System 

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会議名

パシフィコ横浜

発表年月日

2011/12/13

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概要

The elucidation of the translation system is not only one of the most important goal in biology but also, from biotechnological point of view, provides a clue to synthesize large amount of any desired protein from mRNA. Cell-free protein synthesis system was developed as a model to study the mechanism of translation and succeeded to provide a lot of proteins even if its property suppressed cell survival but the attempts to incorporate D-amino acids into proteins with in vitro translation system have been unsuccessful and all D-amino acid-containing peptide and proteins are obtained from posttranslational modification or from nonribosomal synthesis. Here we report that novel method that is able to detect the incorporation of D-amino acid with cell-free protein synthesis system.

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